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» Home » Research Projects » CNCSIS 2010 » Young Teams 127/2010

» Young Teams 127/2010

Contract: 127/2010 - Young Teams, CNCSIS

Titlu proiect: FLUORESCENT SILICA NANOPARTICLES BASED IMAGING TECHNOLOGY - EVALUATION OF TISSUE TOXICITY DUE TO IN VIVO BIOACUMULATION USING BIOCHEMICAL AND MOLECULAR APPROACHES

Summary:

Quantum dots (QDs) represent nano-particles with special optic attributes that stirred the interest of researchers from the domain of biology and medicine. The fluorescent properties they posses comprise the reason they are used as fluorescent probes which are able to detect specific biomarkers in vitro and in vivo, at cellular, tissue and whole-organism level. Silica-based  nanoparticles are being extensively used in the food and cosmetic industries but also in immagistics and targeted transport of therapeutic drugs. Data regarding the toxicological effects of these nanoparticles in vivo and the mechanisms behind their activity are still insufficiently studied. Information published suggest that nanoparticles can induce cytotoxic effects in vitro in various cell types while their in vivo properties are less studied. The aim of this study is to investigate the in vivo effects induced after the intra-peritoneal injection of a fluorescent silica-based nanoparticle suspension in carassius sp. individuals. our goal is to complete a complex and comparative study of different types of tissues obtained after the exposure of fish to silica-based fluorescent nanoparticles. Up to date, no in vivo comparative study has been performed regarding the effects of silica-based fluorescent nanoparticles in tissues where they accumulate after intra-peritoneal injection. Making use of our experience gained in the domain of in vivo analysis of oxidative stress triggered by heavy metals and pesticides poisoning, we shall attempt to identify the cytotoxicity mechanisms induced by these nano-particles at molecular and biochemical level. 

Members of the research team:

- Project manager: Lector Dr. Munteanu Cristina Maria - University of Bucharest – Faculty of Biology - DBBM
- Conf. Dr. Andreea Iren Serban – University of Agronomic Sciences and Veterinary Medicine Bucharest, Facultaty of Veterinary Medicine
- AC. Drd. Radu Mihaela – Research Assistant - University of Bucharest – Faculty of Biology - DBBM
- AC. Drd. Petrache Sorina Nicoleta – Research Assistant - University of Bucharest – Faculty of Biology - DBBM

Activity Reports:

Annual Activity Report 2010

Annual Activity Report 2011

Annual Activity Report 2012

Project work plan:

Working plan of the project Young Teams 127/2010

National and international conferences 2010

  • Stanca L, Petrache SN, Radu M, Munteanu CM, Serban AI, Alterations in the antioxidant defense system in the liver and white muscle of Carassius auratus gibelio exposed to silicon quantum dots. Oral presentation at the “Scientific Research Contributions to Veterinary Medicine Progress” in November 2010, Bucuresti.

National and international conferences 2011

  • Petrache SN, Stanca L, Radu M, Serban AI, Sima C, Zarnescu O, Costache M, Dinischiotu A, Modulation of oxidative status in the red muscle of Carassius auratus gibelio exposed to silicon quantum dots, Toxicology Letters   Official Journal of Eurotox, vol 205S, 2011, pp S224
  • Radu M, Petrache SN, Stanca L, Stan MS, Costache M, Dinischiotu A, Oxidative modification of proteins in different tissues of Carassius auratus gibelio exposed to silicone quantum dots, Toxicology Letters  Official Journal of Eurotox  vol 205S, 2011, pp S224-S225
  • Serban AI, Petrache SN, Stanca L, Dinishiotu A, The oxidative damage induced by silicone quantum dots at kidneys level of  Carassius auratus gibelio, Toxicology Letters  Official Journal of Eurotox, vol 205S, 2011, pp S228. ‚Äč

ISI Journal Articles  2012

National and international conferences 2012

  • Stanca L, Serban AI, Dinischiotu A, Biomarkers of oxidative stress and heat shock protein adaptative modifications in the liver of Cyprinus carpio injected with fluorescent silicone Quantum dots, IUBMB-FEBS 2012 “From Single Molecules to Systems Biology”, FEBS Journal, Volume 279, Issue Supplement s1, p. 207-208
  • Serban A.I., Stanca L., Petrache S.N., Dinischiotu A., Accumulation of Si/SiO2 quantum dots in liver and kidney modulates the antioxidant defense system of Carrasius auratus gibelio, Free Radical Biology and Medicine, Volume 53, Supplement 1, Pages S219, September 2012
  • A. I. Serban, L. Stanca, S. N. Petrache, A. Dinischiotu, The effect of silicone quantum dots on Hsp 60 and Hsp 90 expression in liver and kidney of Carrasius auratus gibelio, 16th SFRRI Biennial Meeting, 6-9 September 2012, London, UK.
  • Loredana Stanca, Anca Dinischiotu - Silicone QDs: a promising tool for biomedicine or danger for the environment? A biochemical perspective, Annual Zoological Congress of “Grigore Antipa” Museum, 21-23 November 2012 Bucharest – Romania, p 205,  ISBN 978-606-92462-7-6
  • Stanca L, Serban AI, Dinischiotu A, Accumulation of Si/SiO2 Quantum Dots In Liver and Kidney Modulates the Antioxidant Defense System of Carrasius auratus gibelio, SFRR-E IUBMB Advanced School  „Dietary f actors and redox signaling” Spetses, Greece, september 24-30, 2012

 

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